Natalya Nikolaevna Ugarova

CHEMICAL ENZYMOLOGY DIVISION
LABORATORY OF PHYSICO-CHEMICAL ASPECTS OF ENERGY BIOCONVERSION Bioluminescence: Principles and Applications

Date of Birth:  November 22, 1936
Education:      1959 - Faculty of Chemistry, Moscow State University
                       Master's Degree in Physical Chemistry

                1966 - Ph.D.in Chemical Kinetics, Moscow State University

                1982 - Doctor  of  Science in Chemical Kinetics,
                       Moscow State University

                1983 - Professor in Chemistry

Research and Professional Experience:

         1959-1970: Junior research worker, Faculty of Chemistry,
                    Moscow State University
         1970-1980: Senior research worker, Faculty of Chemistry,
                    Moscow State University
         1988-present: Head of Laboratory, Moscow State University

Membership  Biochemical Society of Russia
            Scientific Councils of Moscow State University,
            International Advisory Committee on Bioluminescence
            and Chemiluminescence
            Organizing Committees of International
            Conferences on Biocatalysis and Enzyme Engineering

Awards:      ISF (Soros) Grants, 1993-1994
             Grants of Russian Science Foundation,  1989-1994
             Wellcome Trust Collaboration Grant, 1993-1995
             Award of Russian Scientific Foundation, 1994

Publications: more than 200 publications in international and
               russian Journals including 20 books and reviews,
               and more than 20 russian patents

Field of Interest: Enzyme mechanism and enzyme kinetics,
                   Bioluminescence and bioluminescent analysis
                   in medicine, ecology and biotechnology

Topics for scientific lectures:
             Bioluminescence and bioluminescent analysis
             Kinetics and mechanism in firefly bioluminescent
             enzyme system

RECENT PUBLICATIONS:

1. L.Yu. Brovko, O.A. Gandelman and N.N. Ugarova. Firefly bioluminescence. Fluorescence studies of luciferase interactions with oxyluciferin and its analogs. In: Biological luminescence, eds. B. Jezowska-Trzebiatowska, B. Kochel, J. Slawinski and W. Strek. World Scientific. pp. 292-308 (1990).
2. N.N. Ugarova. Research on bio- and chemiluminescence in the Soviet Union. J.Biolum.Chemilum. 6, 137-138 (1991).
3. A.I. Titov, N.A. Romanova, T.M. Danilova, L.Yu. Brovko, N.N. Ugarova and P.I. Tolstykh. Bioluminescent method for assessment of microflora susceptibility to antibiotics. Lab.Delo (USSR) N 10, 61-66 (1990) (in Russian).
4. G.D. Kutuzova, E.A. Skripkin, N.G. Belogurova, G.A. Skryabin, N.N. Ugarova and S.D. Varfolomeev. New type of synthesis induction of recombinant proteins. Low-temperature induction of expession of firefly luciferase genein E.coli. Dokl.AN.SSSR 314, 757-761 (1990) (in Russian).
5. L.Yu. Brovko, I.Yu. Trdatyan and N.N. Ugarova. Optimization of bioluminescent methods of biomass assay. Prikl.biochem.microbiol. (USSR) 27, 134-141 (1991) (in Russian).
6. N.N. Ugarova, Ya.U. Vozny, G.D. Kutuzova and E.I. Dementieva. Bioluminescent assay of b-galactosidase using D-luciferin-O-galactosidase. In: Bioluminescece and Chemiluminescence. Current Status, Proc. VI Int.Symp.Biolum.Chemilum., ed. Ph. Stanley, and L. Kricka, Yohn Wiley & Sons, pp.511-514 (1991).
7. L.Yu. Brovko, N.A. Romanova and N.N. Ugarova. Bioluminescent assay of bacterial intracellular AMP, ADP, and ATP with the use of a coimmobilized three-enzyme reagent (adenylatekinase, pyruvate kinase, and firefly luciferase). Anal.Biochem. 220, 410-414 (1994).
8. O.A. Gandelman, L.Yu. Brovko, A.Yu. Chikishev, A.P. Shkurinov and N.N. Ugarova. Investigation of the interaction between firefly luciferase and oxyluciferin or its analogues by steady state and subnanosecond time-resolved fluorescence. J.Photochem.Photobiol. B:Biol., 22, 203-209 (1994).
9. L.Yu. Brovko, O.A. Gandelman, T.E. Polenova and N.N. Ugarova. Kinetics of bioluminescence in the firefly luciferin-luciferase system. Biochemistry (Moscow) 59, 195-201 (1994).
10. G.D. Kutuzova, O.V. Leont'eva, E.A. Skripkin, and N.N. Ugarova. Kinetics of expression of luciferase genes from the fireflies Photinus pyralis and Luciola mingrelica in Escherichia coli. 1. Kinetics of conversion of recombinant luciferase into enzymatically active conformers. Biochemistry (Moscow) 59, 77-84 (1994).
11. O.V. Leont'eva, G.D. Kutuzova, V.B. Turovetskii and N.N. Ugarova. Kinetics of expression of liciferase genes from the fireflies Photinus pyralis and Luciola mingrelica in Escherichia coli. 2. Role of pH in the biosynthesis of proteins and their transformation into active conformers. Biochemistry (Moscow) 59, 85-88 (1994).
12. N.N. Ugarova. Bioanalytical applications of firefly luciferase (review). Appl.Biochem.Microbiol. (Moscow) 29, 135-144 (1993).
13. N.N. Ugarova, L.Yu. Brovko and G.D. Kutuzova. Bioluminescence and bioluminescent analysis: recent development in the field. Biochemistry (Moscow) 58, 976-992 (1993).
14. A.F. Dukhovich, A.I. Efimov, A.A. Shchegolev, A.V. Levashov and N.N. Ugarova. Firefly luciferase inactivation by long chain choline derivatives. Biochemistry (Moscow), 58, 648-654 (1993) (in Russian).
15. J.H. Devine, G.D. Kutuzova, V.A. Green, N.N. Ugarova and T.O. Baldwin. Luciferase from east european firefly Luciola mingrelica: cloning and nucleotide sequence of the cDNA, overexpression in Esherichia coli and purification of the enzyme. Biochim.Biophys.Acta, 1173, 121-132 (1993).
16. N.A. Romanova, L.Yu. Brovko, M.A. Sepulveda-Bessera and N.N. Ugarova. Change in adenine nucleotide pool in E.coli 1257 cells under low-intensity He-Ne laser illumination. Biochemistry (Moscow), 58, 230-236 (1993).
17. O.A. Gandelman, L.Yu. Brovko, N.N. Ugarova, A.Yu. Chikishev and A.P. Shkurimov. Oxyluciferin fluorescence is a model of native bioluminescence in the firefly luciferin-luciferase system. J.Photochem.Photobiol. B:Biol. 19, 187-191 (1993).
18. G.D. Kutuzova, E.I. Dementieva, T.O. Baldwin and N.N. Ugarova. Recombinant luciferase of firefly Luciola mingrelica. Cloning, superexpression and production of homogeneous enzymepreparation. Biotechnologiya (Moscow) N 5, 43-51 (1992).
19. A.F. Dukhovich, V.M. Morozov and N.N. Ugarova. Effect of substrates on the stability of firefly luciferase incorporated in phosphatidylcholine liposomes. Biochemistry (Moscow), 57, 1179-1182 (1992).
20. L.Yu. Brovko, E.I. Dementieva, E.A. Aronova, P.V. Yevseev and N.N. Ugarova. Bioluminescent analysis of b-galactosidase and its application for immunoassay and DNA-probes. Anal.letters 27, 2949-2959 (1994).
21. A.F. Dukhovich, V.M. Morozov and N.N. Ugarova. Modulation by substrate, adenosine 5'-triphosphate, lipid-protein interactions in firefly luciferin-luciferase system. Biol.membr. (Moscow) 11, 614-619 (1994) (in Russian).